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Molecular Biology


Culture of Animal Cells: A Manual of Basic Technique

Culture of Animal Cells: A Manual of Basic Technique Lowest new price: $66.69
Lowest used price: $11.94
List price: $129.95
Author: R. Ian Freshney

The most complete resource on the techniques, equipment,principles, and practices of animal cell culture

Since publication of the previous edition of this benchmark text, numerous groundbreaking advances have occurred in stem cell research, cloning, tissue engineering, and in vitro toxicity testing. These and other developments have been incorporated into this fully revised and expanded Fifth Edition of Culture of Animal Cells. In addition, to answer the needs of the exponential increase in newcomers to cell culture, particularly in the biopharmaceutical industry, a completely new chapter on training in cell culture technology has been introduced.

The most complete resource on the techniques, equipment, principles, and practices of animal cell culture, this text offers a complete background related to growth of animal cells in culture. Beginning with laboratory design, safety, validation and bioethics, then continuing with preparation of media, primary culture and cell lines, through to characterization and authentication, contamination, specialized techniques, and troubleshooting, the coverage includes:
* An all-new section of training exercises, separated into basic, intermediate, and advanced procedures, cross-referenced to the relevant protocols
* New coverage of stem cells, bioethics, validation, cloning, cell signaling, in vitro toxicity testing, and tissue engineering
* An expanded full-color atlas section, with images of primary culture, cell lines, subculture, differentiation, cancer cells and transformation, three-dimensional culture, contamination, and specialized equipment
* Enhanced treatment of troubleshooting, with full cross-referencing to the relevant protocols and sections of text
* Fully updated references
* The clearest, most consistent presentation of step-by-step protocols available
* Numerous diagrams, photographs, tables, and charts
* Detailed and up-to-date information on reagent preparation and sourcing of materials and equipment, including a fully updated list of suppliers and other resources with Web sites

Indispensable for clinical and biopharmaceutical researchers and scientists, students, trainees, and technicians, this landmark text presents the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells.

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Quantum Field Theory in Strongly Correlated Electronic Systems (Theoretical and Mathematical Physics)

Quantum Field Theory in Strongly Correlated Electronic Systems (Theoretical and Mathematical Physics) Lowest new price: $114.38
Lowest used price: $30.00
List price: $149.00
Author: Naoto Nagaosa
Brand: Naoto Nagaosa

In this book the author extends the concepts introduced in his Quantum Field Theory in Condensed Matter Physics to situations in which the strong electronic correlations are crucial for the understanding of the observed phenomena. Starting from a model field theory to illustrate the basic ideas, more complex systems are analyzed in turn. A special chapter is devoted to the description of antiferromagnets, doped Mott insulators, and quantum Hall liquids from the point of view of gauge theory.

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Archaea: Evolution, Physiology, and Molecular Biology

Archaea: Evolution, Physiology, and Molecular Biology Lowest new price: $132.03
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Brand: Brand: Wiley-Blackwell

Introduced by Crafoord Prize winner Carl Woese, this volume combines reviews of the major developments in archaeal research over the past 10–15 years with more specialized articles dealing with important recent breakthroughs. Drawing on major themes presented at the June 2005 meeting held in Munich to honor the archaea pioneers Wolfram Zillig and Karl O. Stetter, the book provides a thorough survey of the field from its controversial beginnings to its ongoing expansion to include aspects of eukaryotic biology.

The editors have assembled articles from the premier researchers in this rapidly burgeoning field, including an account by Carl Woese of his original discovery of the Archaea (until 1990 termed archaebacteria) and the initially mixed reactions of the scientific community. The review chapters and specialized articles address the emerging significance of the Archaea within a broader scientific and technological context, and include accounts of cutting-edge research developments. The book spans archaeal evolution, physiology, and molecular and cellular biology and will be an essential reference for both graduate students and researchers.

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  • Used Book in Good Condition


Target Discovery and Validation Reviews and Protocols: Emerging Strategies for Targets and Biomarker Discovery, Volume 1 (Methods in Molecular Biology)

Target Discovery and Validation Reviews and Protocols: Emerging Strategies for Targets and Biomarker Discovery, Volume 1 (Methods in Molecular Biology) Lowest new price: $28.96
Lowest used price: $2.77
List price: $229.00
Brand: Brand: Humana Press

Target discovery is a field that has existed for several years but is so vibrant today because of the recent progress in our understanding of the molecular mechanisms of many human diseases and the technical advances in target identification and validation. More sophisticated gene profiling technologies, such as DNA microarrays and serial analysis of gene expression, permit rapid identification of lead targets. Moreover, analysis of gene networks in living organisms allows the identification of target genes that operate in defined physiological pathways. With the sequencing of several genomes completed and the rapidly growing gene expression databases, there is now greater impetus than ever before for in silico discovery of therapeutic targets. Also, recent advances in genetic technologies have increased our ability to generate mouse models for human diseases. The implications of these genetically modified animals in drug development are several, including identification of new drug targets, predicting efficacy, and uncovering possible side effects. Together, these recent technical advances should allow researchers to make the most informed choice early and advance the chosen targets toward clinical studies. Regarding cancers, any difference between a cancer and a normal cell could potentially be exploited as a therapeutic target. The hope is that drugs targeting specific constituents or pathways in cancer cells will provide more effective therapy, either alone or in combination with other currently used anticancer drugs. In addition to drug targets, identifying new target antigens remains as much of a challenge as improving tumor vaccines already in the clinic.

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  • Used Book in Good Condition


CELLS

CELLS Lowest new price: $3.99
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List price: $326.95
Author: Benjamin Lewin
Brand: Brand: Sinauer Associates, Inc.

This important new textbook, designed for advanced undergraduate and early graduate courses in cell biology, covers the structures, organization, growth, regulation, movements, and interactions of cells, with emphasis on eukaryotic cells. Under the direction of Dr. Benjamin Lewin and three expert section editors, each chapter was prepared by top scientists who specialize in the given subject area, and all chapters have been carefully edited to maintain a consistent level throughout the text and to assure that all necessary topics are covered.

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  • Used Book in Good Condition


Molecular Aspects of Myeloid Stem Cell Development

Molecular Aspects of Myeloid Stem Cell Development Lowest new price: $199.99
Lowest used price: $27.00
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Brand: Brand: Springer

A workshop on "MOLECULAR ASPECTS OF MYELOID STEM CELL DEVELOPMENT" was held at the Historic Inns in Annapolis, MD during April 30-May 3, with approximately 70 persons attend­ ing. The enormous success of this meeting was attributable to the recent outburst of information generated from two areas of study: transcriptional regulation of developmentally important myeloid genes and molecular dissection of chromosomal abnormalities in human myeloid leukemias. It was reassuring that studies of normal myelopoiesis and abnormal myelopoiesis, as observed in dys­ plasias and neoplasia, are revealing several interrelated mechan­ isms of gene regulation. However, great challenges await us as we try to determine how these and other mechanisms in this intricate system orchestrate the ultimate fate of cells e. g. proliferation, differentiation or apoptosis. This volume of Current Topics in Micro­ biology encapsulates many of the key aspects of the workshop summarizing research over the past few years on the ontogeny and pathology of myeloid cells. Investigations into the complex process of normal myeloid cell . maturation were first made possible through development of in vitro clonigenic assays. These established the relationships of colony stimulating factors, interleukins and their combinations to the specific fates of myeloid progenitors derived from the bone , marrow and spleen. Molecular studies became feasible, however, through the later establishment of several cell culture systems in­ volving immortalized cells which, under the proper induction, could fairly accurately recapitulate the myelopoietic process.

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Developing Bioinformatics Computer Skills: An Introduction to Software Tools for Biological Applications

Developing Bioinformatics Computer Skills: An Introduction to Software Tools for Biological Applications Lowest new price: $9.80
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List price: $44.99
Author: Cynthia Gibas
Brand: Brand: O'Reilly Media

Bioinformatics--the application of computational and analytical methods to biological problems--is a rapidly evolving scientific discipline. Genome sequencing projects are producing vast amounts of biological data for many different organisms, and, increasingly, storing these data in public databases. Such biological databases are growing exponentially, along with the biological literature. It's impossible for even the most zealous researcher to stay on top of necessary information in the field without the aid of computer-based tools. Bioinformatics is all about building these tools.Developing Bioinformatics Computer Skills is for scientists and students who are learning computational approaches to biology for the first time, as well as for experienced biology researchers who are just starting to use computers to handle their data. The book covers the Unix file system, building tools and databases for bioinformatics, computational approaches to biological problems, an introduction to Perl for bioinformatics, data mining, and data visualization.Written in a clear, engaging style, Developing Bioinformatics Computer Skills will help biologists develop a structured approach to biological data as well as the tools they'll need to analyze the data.

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  • Used Book in Good Condition

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Protein Purification Protocols (Methods in Molecular Biology, Vol. 244)

Protein Purification Protocols (Methods in Molecular Biology, Vol. 244) Lowest new price: $65.10
Lowest used price: $59.96
List price: $229.00
Brand: Brand: Humana Press

The first edition of Protein Purification Protocols (1996), edited by Professor Shawn Doonan, rapidly became very successful. Professor Doonan achieved his aims of p- ducing a list of protocols that were invaluable to newcomers in protein purification and of significant benefit to established practitioners. Each chapter was written by an ex- rienced expert in the field. In the intervening time, a number of advances have w- ranted a second edition. However, in attempting to encompass the recent developments in several areas, the intention has been to expand on the original format, retaining the concepts that made the initial edition so successful. This is reflected in the structure of this second edition. I am indebted to Professor Doonan for his involvement in this new edition and the continuity that this brings. Each chapter that appeared in the original volume has been reviewed and updated to reflect advances and bring the topic into the 21st century. In many cases, this reflects new applications or new matrices available from vendors. Many of these have increased the performance and/or scope of the given method. Several new chapters have been introduced, including chapters on all the currently used protein fractionation and ch- matographic techniques. They introduce the theory and background for each method, providing lists of the equipment and reagents required for their successful execution, as well as a detailed description of how each is performed.

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Skeletal Muscle Mechanics: From Mechanisms to Function

Skeletal Muscle Mechanics: From Mechanisms to Function Lowest new price: $205.45
Lowest used price: $108.00
List price: $459.95
Brand: Walter Herzog

Skeletal Muscle Mechanics: From Mechanisms to Function summarises the variety of approaches used by today's scientist to understand muscle function and the mechanisms of contraction. This book contains research by leading scientists from numerous fields using many different scientific techniques. Topics covered include:
* Cellular and molecular mechanisms of skeletal muscle contraction
* Historical perspective of muscle research
* The newest developments in techniques for the determination of the mechanical properties of single cross-bridges
* Theoretical modelling of muscle contraction and force production
* Multifaceted approaches to determine the in vivo function of skeletal muscle
This state-of-the-art account is written by internationally recognised authors and will be a valuable resource to researchers of biomechanics in sports science and exercise physiology.

"I expect this book to be excellent and timely." Professor R. McNeill Alexander FRS, School of Biology, University of Leeds, UK

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Guidebook to the Cytoskeletal and Motor Proteins

Guidebook to the Cytoskeletal and Motor Proteins Lowest new price: $19.95
Lowest used price: $10.10
List price: $125.00

Since the first edition of Guidebook to Cytoskeletal and Motor Proteins, many new and important discoveries have been made on the structure and function of proteins involved in the microfilament, microtubule and intermediate filament systems in cells. This book collects much of the recent information on this diverse range of eukaryotic proteins, their genes, and the roles they play in cell structure, motor function, and signaling. Like other guidebooks in this series, this volume provides precise and up-to-date information on an important class of biological molecules, enabling both newcomers and specialists to gain access to unfamiliar work. The book begins with a comprehensive look at the structure and function of cytoskeletal actin, tubulin, and intermediate filaments and their associated proteins and continues with coverage of proteins that are involved in new filament systems. A section on molecular motors and associated proteins details all that is currently known about the major motor protein families and associated proteins in the motor complex. As well, a section on organelle structural proteins carefully describes the roles these proteins have in efficient intracellular communication and function. This book is perfect for graduate students and researchers in biochemistry, cell biology and biomedical sciences and will prove itself a useful tool to nonprofessionals interested in molecular biology.


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