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Molecular Biology


HPLC of Peptides and Proteins: Methods and Protocols (Methods in Molecular Biology)

HPLC of Peptides and Proteins: Methods and Protocols (Methods in Molecular Biology) Lowest new price: $154.81
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List price: $199.00
Brand: Brand: Humana Press

The introduction of high-performance liquid chromatography (HPLC) to the analysis of peptides and proteins some 25 years ago revolutionized the biological sciences by enabling the rapid and sensitive analysis of peptide and protein structure through the exquisite speed, sensitivity, and resolution that can be easily obtained. Today, HPLC in its various modes has become the pivotal technique in the characterization of peptides and proteins and currently plays a critical role in both our understanding of biological processes and in the development of peptide- and protein-based pharmaceuticals. The number of applications of HPLC in peptide and protein purification continues to expand at an extremely rapid rate. Solid-phase peptide synthesis and recombinant DNA techniques have allowed the production of large quantities of peptides and proteins that need to be highly purified. HPLC techniques are also used extensively in the isolation and characterization of novel proteins that will become increasingly important in the postgenomic age. The design of multidimensional purification schemes to achieve high levels of product purity further demonstrates the power of HPLC techniques not only in the characterization of cellular events, but also in the production of pepti- and protein-based therapeutics. HPLC continues to be at the heart of the analytical techniques with which scientists in both academia and in industry must arm themselves to be able to fully characterize the identity, purity, and potency of peptides and proteins.

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Protein Engineering: Principles and Practice

Protein Engineering: Principles and Practice Lowest new price: $24.00
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List price: $216.00

PROTEIN ENGINEERING Principles and Practice

Edited by JEFFREY L. CLELAND CHARLES S. CRAIK

Proteins are involved in every aspect of life-structure, motion, catalysis, recognition and regulation. Protein Engineering: Principles and Practice provides a basic framework for understanding both proteins and protein engineering. This comprehensive book covers general, yet essential knowledge required for successful protein engineering, including everything from the fundamentals to modifying existing proteins and developing new proteins.

The book begins by introducing the main concepts of protein engineering, including: understanding protein conformation, comprehending the relationship between protein composition and structure, and potential methods for predicting a protein's conformation.

Other major subjects addressed are:
* Using different host cell expression systems to produce specific proteins
* Protein folding
* Structure and function of proteins in relation to drug design
* Construction of synthetic metal binding sites in proteins
* Manufacture of tissue plasminogen activator
* Generation of therapeutic antibodies

This broad range of topics provides a solid foundation in protein engineering and supplies readers with knowledge essential to the design and production of proteins.

Of primary interest to protein scientists-both students and researchers, in academia as well as industry-Protein Engineering is also extremely useful to chemical engineers, protein chemists, biochemists, and pharmaceutical chemists.

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The Mechanisms of Neuronal Damage in Virus Infections of the Nervous System (Current Topics in Microbiology and Immunology)

The Mechanisms of Neuronal Damage in Virus Infections of the Nervous System (Current Topics in Microbiology and Immunology) Lowest new price: $49.65
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Brand: Brand: Springer

It has been recognized that viruses can induce neuronal damage by a variety of mechanisms. This volume summarizes, for the first time, the various ways that neurons can degenerate under the influence of viral infection, ranging from acute necrosis and virus-induced apoptosis to chronic damage in persistent infections. The mechanisms of neuronal latency are dealt with as well. The volume also includes chapters that review the chronic damage caused by viral proteins that interfere with differentiated functions of the neuron, including signaling by neurotransmitters and signal transduction by trophic factors. Other chapters deal with the controversial role of cellular immune reactions, which may be helpful in controlling infection, but may also be detrimental to the host nervous system. The specific mechanisms of damage involved in retroviral infections and in prion diseases of the nervous system are reviewed, and finally how autoimmune diseases can lead to neuronal damage.

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Enzyme Immunoassays: From Concept to Product Development

Enzyme Immunoassays: From Concept to Product Development Lowest new price: $97.25
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Author: S.S. Deshpande

This unique reference provides a pragmatic approach to the development of successful commercial immunodiagnostic products based on enzyme immunoessay technology. Presenting both the basic and applied principles, Enzyme Immunoassays gathers information on all aspects of this process, from the initial conceptualization to the introduction of the product to the market.


DNA Cloning: A Practical Approach Volume 1: Core Techniques (The Practical Approach Series) (v. 1)

DNA Cloning: A Practical Approach Volume 1: Core Techniques (The Practical Approach Series) (v. 1) Lowest used price: $3.84
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Part of a four-volume set on DNA cloning, this particular book describes all of the necessary background and detailed laboratory methods for the core techniques used in every molecular biology laboratory.

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Liposomes: A Practical Approach (Practical Approach Series)

Liposomes: A Practical Approach (Practical Approach Series) Lowest new price: $123.99
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Brand: Brand: Oxford Univ Pr

Since the first edition of "Liposomes - A Practical Approach" was published in 1990, Liposome technology has become a highly successful and rapidly developing area of preclinical and clinical research. During the 90's, a number of liposome-based drugs reached the market in the U.S. and Europe and several new aspects of liposome research have been discovered. As a consequence there has been an explosion of new procedures and techniques in the field. Reflecting both the significant changes in the field and the new discoveries, this new edition of Liposomes contains a combination of chapters from the 1st edition which have been significantly updated, and several new chapters.

Each chapter is an outstanding contribution from leading experts in the field, and the book as a whole encompasses all key areas of liposome technology. The first half of the book addresses a number of essential experimental protocols such as the preparation, characterization, and storage of liposomes. Also included are methods for drug encapsulation into liposomes, modification of the liposomal surface to control drug behaviour in biological environments, long-circulating liposomes and the use of cationic liposomes as transfection vectors. The second half of the book discusses specific areas of liposome research and application, such as pH-sensitive liposomes, liposomes for diagnostic imaging, liposomal DNA vaccines, isothermic titration calorimetry of liposomes and vesicular phospholipids gels.

This new edition of Liposomes is an essential tool for all experienced liposomologists and the perfect introduction for researchers new to the field.

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Arabidopsis Protocols, 2nd Edition (Methods in Molecular Biology)

Arabidopsis Protocols, 2nd Edition (Methods in Molecular Biology) Lowest new price: $13.60
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For several decades, Arabidopsis thaliana has been the organism of choice in the laboratories of many plant geneticists, physiologists, developmental biologists, and biochemists around the world. During this time, a huge amount of knowledge has been acquired on the biology of this plant species, which has resulted in the development of molecular tools that account for much more efficient research. The significance that Arabidopsis would attain in biological research may have been difficult to foresee in the 1980s, when its use in the laboratory started. In the meantime, it has become the model plant organism, much the same way as Drosophila, Caenorhabditis, or mouse have for animal systems. Today, it is difficult to envision research at the cutting edge of plant biology without the use of Arabidopsis. Since the first edition of Arabidopsis Protocols appeared, new developments have fostered an impressive advance in plant biology that prompted us to prepare Arabidopsis Protocols, Second Edition. Completion of the Arabidopsis genome sequence offered for the first time the opportunity to have in hand all of the genetic information required for studying plant function. In addition, the development of whole systems approaches that allow global analysis of gene expression and protein and metabolite dynamics has encouraged scientists to explore new scenarios that are extending the limits of our knowledge.


The Polymerase Chain Reaction

The Polymerase Chain Reaction Lowest new price: $45.95
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Brand: Kary B Mullis

James D. Watson When, in late March of 1953, Francis Crick and I came to write the first Nature paper describing the double helical structure of the DNA molecule, Francis had wanted to include a lengthy discussion of the genetic implications of a molecule whose strucĀ­ ture we had divined from a minimum of experimental data and on theoretical arguĀ­ ments based on physical principles. But I felt that this might be tempting fate, given that we had not yet seen the detailed evidence from King's College. Nevertheless, we reached a compromise and decided to include a sentence that pointed to the biological significance of the molecule's key feature-the complementary pairing of the bases. "It has not escaped our notice," Francis wrote, "that the specific pairing that we have postulated immediately suggests a possible copying mechanism for the genetic material." By May, when we were writing the second Nature paper, I was more confident that the proposed structure was at the very least substantially correct, so that this second paper contains a discussion of molecular self-duplication using templates or molds. We pointed out that, as a consequence of base pairing, a DNA molecule has two chains that are complementary to each other. Each chain could then act ". . . as a template for the formation on itself of a new companion chain, so that eventually we shall have two pairs of chains, where we only had one before" and, moreover, " ...


Biochemical Adaptation: Mechanism and Process in Physiological Evolution

Biochemical Adaptation: Mechanism and Process in Physiological Evolution Lowest new price: $48.84
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Author: Peter W. Hochachka
Brand: Peter W Hochachka

The study of biochemical adaption provides fascinating insights into how organisms "work" and how they evolve to sustain physiological function under a vast array of environmental conditions. This book describes how the abilities of organisms to thrive in widely different environments derive from two fundamental classes of biochemical adaptions: modifications of core biochemical processes that allow a common set of physiological functions to be conserved, and "inventions" of new biochemical traits that allow entry into novel habitats. Biochemical Adaptation: Mechanisms and Process in Physiological Evolution asks two primary questions. First, how have the core biochemical systems found in all species been adaptively modified to allow the same fundamental types of physiological processes to be sustained throughout the wide range of habitat conditions found in the biosphere? Second, through what types of genetic and biochemical processes have new physiological functions been fabricated? The primary audience for this book is faculty, senior undergraduates, and graduate students in environmental biology, comparative physiology, and marine biology. Other likely readers include workers in governmental laboratories concerned with environmental issues, medical students interested in some elements of the book, and medical researchers.

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Plant Chromosomes

Plant Chromosomes Lowest new price: $158.93
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List price: $209.95
Author: Archarna Sharma
Brand: Brand: CRC Press

The 1980s and 1990s have brought with them progress in plant chromosomal research. The chromosome has been clarified in detail, enabling identification of even gene sequences at the microscopic level, which has aided in the analysis of biodiversity. Knowledge of chromosome structure has played a crucial role on the improvement of crop species and has far-reaching implications. The manipulation and engineering of chromosomes involves novel methods, combining conventional and modern techniques of biotechnology. A working knowledge of such techniques is useful for students and researchers and the plant system, because of its totipotency, requires special treatment. This treatise covers methods involved in the study of evolution, biodiversity, and chromosome manipulation and engineering.

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